High-Throughput Screening of Ion Channel Variants Using Automated Patch Clamp Recordings in Assay Ready Cells
In the ground-breaking study summarized here, the authors presented a method for the high-throughput evaluation of the functional consequences of KCNQ2 ion channel variants.1 The technique was used to characterize 39 previously unstudied epilepsy-associated Q2 variants and their responses to treatment with a candidate therapeutic.
Read MoreScalable Protein Production Using Flow Electroporation
Many transient gene expression (TGE) methods produce insufficient protein quantities for full use within biotherapeutic and vaccine development pipelines.
Read MoreRapid Production of Cells for Screening Voltage-Gated Ion Channels in Automated Electrophysiology Assays Using the MaxCyte® STX™ Scalable Transfection System
Comprising a family with hundreds of members, ion channels represent one of the largest classes of drug targets. Recent advances in automated electrophysiology instrumentation have led to the routine implementation of high throughput assays for screening ion channel modulators.
Read MoreLiterature Review: CHO versus HEK Cell Glycosylation
Choosing a host system for the expression of recombinant proteins should be carefully evaluated prior to the initiation of any biotherapetuic development programs. S
Read MoreKeys to Successful Cell-Based Assay Development with the MaxCyte® STX™ Scalable Transfection System
The MaxCyte® STX™ Scalable Transfection Sys tem allows users to transfect up to 1×1010 cells with single or multiple plasmids encoding GPCRs, ion channels and other targets.
Read MoreGram Scale Transient Antibody Production and Stable Cell Line Generation Using Flow Electroporation™ Technology
In recent years researchers have turned to transient gene expression (TGE) as an alternative to CHO stable cell line production for early stage antibody development (1,2).
Read MoreFlow Electroporation for Vaccine Development and Production: From Subunit Vaccines to Ex Vivo Immunotherapy
MaxCyte flow electroporation is a universal, clinically validated transient transfection platform for rapid, high-quality cell transfection.
Read MoreFlow Electroporation Capabilities and Case Studies: Rapid GPCR Screening and Functional Ion Channel Assays.
MaxCyte’s proprietary flow electroporation technology has been successfully applied in ex vivo cell therapy (1) and drug discovery pipelines where reproducibility, efficiency and the need for increased cell numbers are critical.
Read MoreElectroporation Enables Oligonucleotide Delivery to Regulate Gene Expression in Friedrich’s Ataxia Patient-Derived Cells
Oligonucleotide drugs are an up-and-coming area of development that presents many possibilities for the personalized treatment of rare diseases.
Read MoreClinical-scale NK Cell Engineering Using mRNA Electroporation for the Development of Novel Cell-based Mono- and Combination Immunotherapies.
The ability to engineer sensitive cells, such as primary and stems cells, with high efficiency and cell viability is key to harnessing late-breaking scientific discoveries and exploring novel therapeutic approaches
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